APOLLO'S HGF hcx

Apollo's human cell expressed HGF is the worlds first HGF that has the post translational modifications (PTM's) that are found in the human body. HGF contains both N and O linked oligosaccharides and Apollo's HGF contains both of these, unlike products produced in E- coli or Baclovirus that would not have these PTM's.

Hepatocyte Growth Factor/Scatter Factor (HGF/SF) was originally identified and characterized as two different factors, one with growth-stimulating activity (HGF) and the other with scatter factor activity (SF). These two activities were subsequently ascribed to the same factor. HGF/SF is a mesenchymally derived, heparin-binding glycoprotein with mitogenic, motogenic, and morphogenic effects on a variety of cells. In vivo, HGF/SF exists in two forms, a biologically inactive monomeric molecule (e.g. in human placenta) and a biologically active heterodimer (e.g. in human serum). HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

In order to determine the activity of HGF hcx, we assayed its ability to induce dissolution of cell-cell contacts between carcinoma cells and their subsequent scattering.

Bioactivity Results

Scc9 C3 cells (Stably over-expressing cortactin) were grown into colonies, the media removed and replaced with media containing HGF (10 µg/mL) for 16 h. Cell scattering was monitored by photography using a Leica DFC280 camera with Leica Firecam software (version 1.9). Results are seen in Figure 1.

Figure 1

Scc9 C3 cells were serum starved for 3 hours and then stimulated with HGF (10 µg/mL) for the time periods indicated. Cell lysates were prepared and western blotting was undertaken as indicated in Figure 2.

Figure 2

Further reading:

• Download brochure : Apollo's hcx proteins for cancer research

View relevant products:

• » HGF

References

For a review of HGF and its role in Cancer, see:

1. Jiang et al. (2005) Crit Rev Oncol Hematol. 53(1) (35-69)

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