APOLLO'S G-CSF and SCF vs E.Coli

Apollo's human cell expressed G-CSF and SCF promote greater stem cell expansion than E. coli expressed cytokines

G-CSF and SCF in combination can stimulate granulocytic differentiation and proliferation of CD34⁺ human haemaopoietic stem cells. G-CSF is glycosylated with one O-linked oligosaccharide. SCF is heavily glycosylated with up to 50% of its molecular weight consisting of carbohydrate.

Apollo's G-CSF^hcx and SCF^hcx were evaluated in a colony-forming assay, whereby CD34⁺ cells were inoculated into semisolid cultures of methylcellulose with G-CSF and SCF and grown for 14 days. Apollo's glycosylated G-CSF^hcx and SCF^hcx produced more and larger colonies, compared to commercially available non-glycosylated cytokines produced in E. coli as illustrated below.

When used in combination to expand haemtopoietic stem cells, Apollo's glycosylated G-CSF^hcx and SCF^hcx also promoted significantly more cell proliferation (up to 53% higher: p<0.0001) than E. coli expressed G-CSF and SCF.

This study shows that hcx^TM G-CSF and SCF used together form a powerful combination for inducing stem cell expansion in vitro. As proteins are expressed in human cell lines this gives them unique potential for clinical therapies, where there is a growing preference for eliminating non-human products.

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