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ELISA Kits

Human cell expressed proteins with human post-translational modifications

Apollo Cytokine Research offers ELISA kits with recombinant human protein standards expressed from human cells because species-to-species variation in post-translational modifications (PTMs) can affect ELISA immunoreactivity.

There is a lack of research identifying how these PTM differences affect ELISA immunoreactivity. Commercially available ELISA kits use non-human cell expressed protein standards, particularly E. coli, and antibodies derived against these proteins. While some kits use alternatives to E. coli standards, such as insect and CHO derived proteins, few, if any, ELISA kits utilise human cell expressed recombinant proteins.

Research undertaken at Apollo has identified differences in immunoreactivity of human cytokines and growth factors expressed in human cells, compared to the same proteins expressed in non-human cells. More >>

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ELISA Kits:  A-H   I-L   M-Z
Protein  Species  Source  % Carbohydrate
 G-CSF AccuKineTM ELISA Kit  Human  Human  0-10
 GM-CSF AccuKineTM ELISA Kit  Human  Human  5-50
 IL-2 AccuKineTM ELISA Kit  Human  Human  0-15
 IL-3 AccuKineTM ELISA Kit  Human  Human  0-40
 IL-4 AccuKineTM ELISA Kit  Human  Human  0-25
 IL-6 AccuKineTM ELISA Kit  Human  Human  0-20
 IL-10 AccuKineTM ELISA Kit  Human  Human  <5
 Lymphotoxin-alpha/TNF-beta AccuKineTM ELISA Kit  Human  Human  0-25
 TNF-alpha AccuKineTM ELISA Kit  Human  Human  <5
 TNF-beta/Lymphotoxin-alpha AccuKineTM ELISA Kit  Human  Human  0-25
 VEGF-165 AccuKineTM ELISA Kit  Human  Human  0-20

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