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Recombinant Human TNF RI - Fc Chimera

Apollo's purified TNF RI - Fc Chimera^hcx has N-linked and probably O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further slight drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Apollo's TNF RI - Fc Chimera^hcx consists of 5-20% carbohydrate by weight.

Densitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Apollo's hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. For more details, please see product sheet.

1. Chen G & Goeddel DV (2002) Science 296: 1634-5.

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For more information on this protein, please click on the protein name below to download a PDF version of the product sheet.

TNF RI - Fc Chimera:
Protein	Species	Source	% Carbohydrate	Catalogue No.	Size	Price (USD)
TNF RI - Fc Chimera	Human	Human	5-20	9010G	20 ug	$240.00	Add to Cart

For further technical information about Apollo's range of hcx proteins, please click here.