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Recombinant Human IL-5

Apollo's purified IL-5^hcx has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Apollo's IL-5^hcx consists of 10-35% carbohydrate by weight.

Densitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Apollo's hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. For more details, please see product sheet.

1. Mordvinov VA & Sanderson CJ (2001) Arch Immunol Ther Exp. (Warsz) 49: 345-51.
2. Kodama S, et al. (1993) Eur J Biochem. 211: 903-8.

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For more information on this protein, please click on the protein name below to download a PDF version of the product sheet.

IL-5:
Protein	Species	Source	% Carbohydrate	Catalogue No.	Size	Price (USD)
IL-5	Human	Human	10-35	4005C	5 ug	$255.00	Add to Cart
IL-5	Human	Human	10-35	4005D	10 ug	$460.00	Add to Cart

For further technical information about Apollo's range of hcx proteins, please click here.