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Recombinant Human GM-CSF hcx

Apollo's GM-CSFhcx contains N-linked and probably O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further small drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Apollo's GM-CSFhcx consists of 5-50% carbohydrate by weight.

Click here to enlarge GM-CSF densitometry imageDensitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

GM-CSF 1D gel image1D SDS-PAGE of Apollo's hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. For more details, please see product sheet.

1. Hamilton JA & Anderson GP (2004) Growth Factors 22: 225-31.

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For more information on this protein, please click on the protein name below to download a PDF version of the product sheet.

GM-CSF:
Protein Species Source % Carbohydrate Catalogue No. Size Price (USD)  
GM-CSF Human Human 5-50 1002C 5 ug $480.00 Add to Cart Click here to view cart
GM-CSF Human Human 5-50 1002D 10 ug $720.00 Add to Cart Click here to view cart
GM-CSF Human Human 5-50 1002G 20 ug $1080.00 Add to Cart Click here to view cart
GM-CSF AccuKineTM ELISA Kit Human Human 5-50 E1002F Kit $895.00 Add to Cart Click here to view cart

For further technical information about Apollo's range of hcx proteins, please click here.